Toxo Genetic Marker Table

                                             

                                                                                                                                                Markers
Markers AKA Chrm Chr Position 10XScaffold # 10xPosition Primers 1 Primers 2 PCR(bp) Ann temp (C) Restr Enz 1 Digest Condition1 Type I      II     III Restr Enz 2 Digest Condition2 Type I      II    III
AK3 TGG994723-750 Ia 1 TGG_994723 749-1528 TGG_994723-750F 5’-ACAACCACATTCCAAAAAGTA-3’ TGG_994723-750R 5’-CCCAGAGAAACCACTGTATG-3’ 780 52 BstUI NEB2            60C  3% gel 1            2            2      
AK150 AK150B Ia 2 TGG_994723 167133-167692 AK150B-F 5'-ACGCGCATATGCTTGTAAGA-3' AK150B-R 5'-ACAAGAGAGGGAACGCCAGA-3' 560 55 BbvI NEB2            37C  3% gel 1            2            2      
M48   Ia 3 TGG_994723 330799-330983 48-1F 5’-AACATGTCGCGTAAGATTCG-3’ 48-2R 5’-CTCTTCACTGAGCGCCTTTC-3’ ~ 210 55 MicroS                             4% gel 1            2            2      
AK158   Ia 4 TGG_994723 424334-424555 AK158-F  5'-CCTCGTCCTGTTCAATGAC-3' AK158-R 5'-CAGAAGGGAGAGCACAGTT-3' 351 55 BstF5I NEBSE-Y      65C  3% gel 1            2            1      
AK4 TGG994723-480425 Ia 5 TGG_994723 480533-481356 TGG_994723-480425F 5'-TTTCGCTCGATACTGTGAG-3' TGG_994723-480425R  5'-TTGCAAATACTCGAGTTGG-3' 824 52 BstNI NEB2  BSA   60C  3% gel 1            2            2      
AK1 TGG994723-1054747 Ia 6 TGG_994723 1054695-1054894 TGG_994723-1054747F 5'-ATTCTCTTGGTGACCTGAAGAGC-3' TGG_994723-1054747R 5'-AGGTAAGACGAGGTGAATCAAAA-3' 200 55 HinfI NEB2            37C  3% gel 1            2            2      
AK6 TGG994726-338890 Ia 7 TGG_994726 338707-339072 TGG994726-338890F 5'-ATCTCAGTCGCGGTTTTT-3' TGG994726-338890R-5'-ATTACTCTTTCACACGCTGTC-3' 366 52 AluI NEB2            37C  3% gel 1            2            1      
AK7 TGG994726-337608 Ia 8 TGG_994726 337435-337786 TGG994726-337608F 5'-TATTCTTCCTCCATGCATTC-3' TGG994726-337608R 5'-GAAGAGAAACTGGGAAACCT-3' 352 52 MboI NEB3            37C  3% gel 1            2            2      
AK2 TGG994726-19617 Ia 9 TGG_994726 19360-19968 TGG994726-19617F 5'-CATGGCTTCGAGAACACTCA-3' TGG994726-19617R 5'-CGTCTGCTAGAATGCCAAGA-3' 609 55 NheI NEB2  BSA   37C  3% gel 1            2            1      
AK15 TGG995280-5 Ib 1 TGG_995280 63001-63758 TGG995280-5'F  5'-AGGGTAGCACTGGTCTGTC-3' TGG995280-5'R  5'-AGTTTGGCACTGTGTCTGTT-3' 758 52 DdeI NEB3            37C  3% gel 1            2            2      
MIC4   Ib 2 TGG_995280 533027-533404 MIC4-F2  5'- CGGTACGGCATTACTGGATT -3' MIC4-R2  5'-AGCGATAAAGCCATCACCAC -3' 378 55 Mbo II NEB2            37C  3% gel 1            2            2      
ACT1   Ib 3 TGG_995280 997399-997844 ACT1-F  5’-GGAAATGAAGGTCGGGAGCT-3’ ACT1-R  5’-CCGCCTGAAAATTGAGAGGG-3’ 446 55 Bsm FI NEB4  BSA   65C  2% gel 1            2            2      
c4-5   Ib 4 TGG_995280 1009966-1010344 C4-5F    5’ – CTTGCGTGTTGCCTCATAGA-3’ C4-5R   5’ -  CGCCGTCAGTCTTTTCAAG –3’ 377 55 Taq I NEBU  BSA   65C  2% gel 1            2            2      
AK16 100121265-Ib Ib 5 TGG_995280 1534989-1535337 100121265-IbF 5'-TGCGTCTTTCTCAGAGTTG-3' 100121265-IbR 5'-CAGGAGAAAAGGTAACTGGA-3' 349 53 HinfI NEB2            37C  3% gel 1            2            2 BbvI NEB2            37C  3% gel 1            2            1
AK94 AK94A Ib 6 TGG_995334 318006-318566 AK94A-F 5'-CTGCGGAGCGAGTCTTCT-3' AK94A-R 5'-AAGACGGTCCCTCTCTCC-3' 561 55 HpyCH4IV NEB1            37C  3% gel 1            2            1      
c22-8 c22 Ib 7 TGG_995334 103084-103604 C22-8F: 5'-TCTCTCTACGTGGACGCC-3' C22-8R: 5'-AGGTGCTTGGATATTCGC-3' 521 55 Bsm AI NEB3            55C  2% gel 1            2            2 Mbo II NEB2            37C  3% gel 1            1            2
G11L-T7   II 1 TGG_994725 32782-33326 G11L-F1  5’ – AGTGCGTGGCGCTGTAAC – 3’ G11L-R1  5’ – CTTTTGCATGACACTGCC – 3’ 545 55 Bsm AI NEB3            55C  3% gel 1            1            2      
AK57   II 2 TGG_994740 608195-608585 AK57-F'GCGTCGTAGTCCCCTCAT-3' AK57-R'GCTGTCCCACACGAGAAA-3' 275 55 BcgI NRBU  SAM  37C  2% gel 1            2            2      
AK56 RC14 II 3 TGG_994740 286628-287150 AK56F 5'-GTCCTTTTCCCCACTGCTTT-3' AK56R 5'-GATGGATTCCGCGAGTTTTA-3' 523 55 MfeI NEB4            37C  3% gel 1            2            1      
RC3 6xTGG9914-5 II 4 TGG_994740 3755-4418 TGG9914 5’-5F  GAGCTGCGGATTTAAGTTGC-3' TGG9914 5’-5R  TTTCGTCACTCATGCCTCTG -3' 679 55 MnII NEB2  BSA   37C  2% gel 1            2            1      
L351   II 5 TGG_995360 1341959-1342482 L351-F1  5’-TCACATACGAATTTCATCTCCG-3’ L351-R1  5’-GAACGGCTGAATTTGACTCTC-3’ 524 55 Nla III NEB4  BSA   37C  3% gel 1            2            2 Mae III 2x Buffer       55C  2% gel 1            2            1
KT-L379A L379B II 6 TGG_995360 1178102-1178591 L379B 995360F 5'-TTTATAGGGAGTGCCGCTGTGGA-3' L379B 995360R 5'-AGCCACTGAATCTCGAATACTCGTCTC-3' 489 62 XhoI NEB2  BSA   37C  1% gel 1            1            2      
W35487   II 7 TGG_995360 633273-633482 W35487-F 5'- GGTTCACTGGATCTTCTCCAA -3' W35487-R 5'- AATGAACGTCGCTTGTTTCC -3' 243-249 55 Alu I NEB2            37C  2% gel 1            1            2 Bsg I NEB4  SAM   37C  2% gel 1            2            2
L31-T3 L31 II 8 TGG_995360 55961-56153 L31-F1x  5'-TTCCTCGCTGGACACTGC-3' L31-R1x  5'-TCAGCAGAGCCGACGAGT-3' 192 55 MicroS                             3% gel 1            1            2      
AK17  6xTGG9931-5 III 1 TGG_995293 5675-6364 TGG9931-3F5’-GTACTGAGCCGTAGACATCG -3’ TGG9931-3R 5’-CTCATGCCTTTCAAAAACAG -3’ 690 55 BstNI NEB2  BSA   60C  3% gel 1            2            1      
AK95 AK95A III 2 TGG_995298 331905-332574 AK95AF 5'-ACTGTCCGCTTTCTGCTG-3' AK95AR 5'-GTCGCACCTACTTCAACTG-3' 670 55 BseRI NEB2            37C  3% gel 1            2            2      
AK119 AK119B III 3 TGG_995298 76402-76986 AK119BF 5'-TGAATTCGTCTGCCAGTAA-3' AK119BR 5'-GCAGATCCGCATTTTAGA-3' 585 55 AvaII NEB4            37C  3% gel 1            2            1      
c29-2   III 4 TGG_995365 285620-286065 c29-2F 5’-AGTTCTGCAGAGTGTCGC-3’ c29-2R 5’-TGTCTAGGAAAGAGGCGC-3’ 446 55 HpyCH4 IV NEB1            37C  2% gel 1            1            2 Rsa I NEB1            37C  2% gel 1            2            1
AK96   III 5 TGG_995365 723948-724600 AK96-F 5'-TGATGATTTCAAACGGTAAA   -3' AK96-R 5'- TTCAAGTCTAGATTATATTGAAACG-3'
-3'		 650 53 BstUI NEB2  BSA   60C  2% gel 1            2            1      
cB21-4 cB21 III 6 TGG_995365 1143646-1144147 cB21-4F 5’-CCAGGTGTTTCGATATTGAT-3’ cB21-4R 5’-GCCTGTGTGGTGTTCGAATC-3’ 502 55 Hae III NEB2            37C  2% gel 1            2            3 HpyCH4 IV NEB1            37C  2% gel 1            2           1
AK151   III 7 TGG_995365 1383739 -1384082 AK151F 5'-AGAGAAGACGGGAGTCTCA-3' AK151R 5'-CGTGACCTTCAGATCCACT-3' 344 55 Hpy188III NEB4  BSA   37C  3% gel 1            2            1      
AK97 AK97C III 8 TGG_995365 1653610-1654325 AK97CF 5'-CTCCATCCTCTTTTGTTCCT-3' AK97CR 5'-AAGACGGAAACACAAAATGT-3' 716 55 BsaJI NEB2            60C  3% gel 1            2            1      
AK39  6xTGG9828-5 III 9 TGG_994749 299969-299286 TGG9828-5F 5’-TAGCTCACGTGGGAAAATTA-3' TGG9828-5R 5’-AGCTCATAGAGGACCTTTGG-3 684 55 AlwNI NEB4            37C  3% gel 1            2            2      
AK118 AK118A III 10 TGG_994749 17308-18042 AK118A-F 5'CGCAACTTCTATTCGATGC-3' AK118A-R5'-AGATATATCGCCAACGAGAA-3' 735 50 RsaI NEB1            37C  2% gel 1            2            2      
ST7A   III 11 TGG_995333 70611-71291 ST7A-F 5'-GGTGAGAGTCAGGACAAACT -3' ST7A-R 5'-TCTTCCGCTGAACAACAC -3' 680 55 Sau3AI NEBU  BSA   37C  2% gel 1            2            2      
cB8-9   IV 1 TGG_995351 4874-5112 cB8-9F1  5’ – CGCAGAGCGTGAAGTCATAG – 3’ cB8-9R1  5’ – TACGACACGACGTAGCAAGG – 3’ 428 55 Hpa II NEB1            37C  2% gel 1            1            2      
c18-TA8   IV 2 TGG_995351  10599-11109 C18TA8-R  5’ – GAGTCCCAGCTGTCATACCG-3’ C18TA8-F  5’ – CACCCAGCTTCACACCTCAG-3’ 511 55 Mnl I NEB2  BSA   37C  2% gel 1            1            2      
cA5-22   IV 3 TGG_995330  2194-2633 cA5-22R  5’ – CAGCGCGGTACACAAACTC – 3’ cA5-22F  5’ – TGTTTTTGCTACGGATTGCTC – 3’ 442 55 Hpa II NEB1            37C  2% gel 1            1            2      
AK98 AK98B IV 4 TGG_995331 1429581-1430134 AK98-F 5'-ATCCAGCCATGTAACGATAA-3' AK98-R 5'-TTGGAGTAAAGCTATTTCACG-3' 552 55 TaqI NEBU BSA    65C  2% gel 1            1            2      
BSR4   IV 5 TGG_995331  1106385-1107099 BSR4-F  5’-CAAGGCTAACCGTAGCGGGT-3’ BSR4-R  5’-CTCTTCAGGAACTTGGGGAA-3’ 715 55 Ase I NEB3            37C  2% gel 1            1            2 Sau3A I NEBU   BSA  37C 2% gel 1            1            2
M33   IV 6 TGG_995331  854415-854584 33-2R 5’-TCTTTTCTCCCCTTCGCTCT-3’ 33-1F 5’-TACGCTTCGCATTGTACCAG-3’ ~170 55 MicroS 10% PAGE in 0.5X TBE 1            1            2      
Bc18U Bc18 IV 7 TGG_995331  640279-640845 BC18U-F  5’-TTGGTACACTTGCCCTGTCG-3’ BC18U-R  5’-GCACATGGTCTTGCTGCAGC-3’ 566 55 Bsl I NEB3            55C  2% gel 1            1            2      
Bs226 226 IV 8 TGG_995331 639623-640228 226-F  5’-CAGTTCGCTCATCTACCATC-3’ 226-Rx  5’-TTTTCACTCAAGCTCCCCTC-3’ 607 55 Mae III 2x buffer        55C  2% gel 1            1            2      
AK18 6xTGG9355-391 IV 9 TGG_995331 377123-377395 TGG9355_391F 5’-AGCACAGTCCCTTGATGACC  -3’ TGG9355_391R 5’- GAAGCAACTGGGGAGGAAAT -3’ 273 55 Cac8I NEB3            37C  3% gel 1            1            2      
KT-c20R c20 IV 10 TGG_995331 81049-81603 KT-C20R F2  5’-TTCGAAAGAGAAGTAACCGGCGTGT-3’ KT-C20R R2  5’-AGACGCCTGGCGGGTTGCC –3’ 554 62 Bcc I NEB1  BSA   37C  2% gel 1            1            2      
AK88 TGG_995281-B IV 11 TGG_995281 509245-509608 TGG_995281-BF  5'-GTAGCGTAAACGCCTGAGT-3' TGG_995281-BR  5'-CTCTTCCAACTGACGAATG-3' 340 53 FokI NEB4            37C  3% gel 1            1            2      
AK89 TGG_995281-A IV 12 TGG_995281 506779-507191 TGG_995281-AF  5'-GCTGACGTTGCAGAGAGA-3' TGG_995281-AR  5'-CTCTCCGATGCACCTAAAT-3' 360 53 MfeI NEB4            37C  3% gel 1            1            2      
AK99   IV 13 TGG_995281 2608-2984 AK99C- F 5'- TCGTCGTCTTCTCAGTATCC-3' AK99C-R 5'-TAAAGGAATCCCAGCGAAG-3' 377 50 MspAI NEB4  BSA   37C  2% gel 1            1            2      
AK120   IV 14 TGG_994761 177798-178304 AK120F 5'-GAGAGAAGAGCGAAGGAGAC-3' AK120R 5'-CTCTCCCCCAAGGACCTC-3' 507 55 BgI III NEB3            37C  3% gel 1            1            2      
AK19 6xTGG9448-3 IV 15 TGG_994761 330781-331449 TGG9448-3F 5’-GAGCGCAGTGAATATGAGAC -3’ TGG9448-3R 5’-AAACATGACATGGCAAGAAC-3’ 669 55 HinfI NEB2            37C  3% gel 1            1            2      
ST12A   IV 16 TGG_994707 83204-83777 ST12A-F  5'-GAACTTCCGCCTCACACA-3' ST12A-R 5'- GTTTCTTGCGGGCAGTGT -3' 572 55 AciI NEB3            37C  2% gel 1            2            1      
AK85 TGG_995369-B IX 1 TGG_995369 32658-32925 TGG_995369-BF  5'-TCGCAGTAAGAGTGGAGATA-3' TGG_995369-BR  5'-TCTCCCACCAGTAAACCAC-3' 255 53 SfcI NEB4  BSA   37C  3% gel 1            2            2      
AK86 TGG_995369-A IX 2 TGG_995369 14710-15129 TGG_995369-AF 5'- TTAAACTCTGACACTCTTCTGC-3' TGG_995369-AR 5'-TCTCTTCAGAGAAATAGCAACC-3' 410 53 BbvI NEB2            37C  3% gel 1            2            2      
AK87 TGG_994713-A IX 3 TGG_994713 43615-44104 TGG_994713-AF  5'-AAAAGACCAAGGGGAAAGT-3' TGG_994713-AR  5'-CAGATGAGTCACCACTAGCA-3' 460 53 TaqI NEBU  BSA   65C  3% gel 1            2            1      
AK54 RC13 IX 4 TGG_995367 1847561-1848160 AK54-F 5'CAGATCCTCGAAGAGTGGT -3' AK54-R 5'CTTCAAGGATTCTTCGTCAG-3' 503 55 HaeIII NEB2            37C  2% gel 1            2            1      
BTUB TUB2 IX 5 TGG_995367 1372413-1372823 Btb-F  5’-GAGGTCATCTCGGACGAACA-3’ Btb-R  5’-TTGTAGGAACACCCGGACGC-3’ 411 55 BsiEI NEB2  BSA   60C  2% gel 1            2            2 TaqI NEBU  BSA   65C 3% gel 1            2            1
AK31 6xTGG9932-5 IX 6 TGG_995367 1033351-1034070 TGG9932-5F 5’-GGTTCGTGCTTCCTCTTCAG -3’ TGG9932-5R 5’- GTCTTGTTCTGCCAGCCTTC-3’ 757 55 AfI III NEB3  BSA   37C  3% gel 1            2            2      
AK55 RC12 IX 7 TGG_995367 612122-612512 AK55-F5'-AGTAGCAAGCATCAGTGGAG-3' AK55-R 5'- AATCGAAACCCTTTTCTTG-3' 391 55 PstI NEB3  BSA   37C  2% gel 1            2            1      
RC1 6xTGG9932-3 IX 8 TGG_995367 63044-63883 RC1F-5'TCACCATTTGCTGCTGAAAG-3' RC1R'-5'GGTGAGAGTCCGTGGTGTTT-3' 796 55 Fnu4HI NEB4            37C  2% gel 1            2            1      
L375   IX 9 TGG_994720 40328-40799 L375-F1 5’-GAAGGTTCCGATCCATGTTG-3’ L375-R1 5’-CGACCAAGCAACTGTCTCAA-3’ 472 55 Mbo I NEB3            37C  2% gel 1            2            1      
AK58 RC16 IX 10 TGG_994720 521698-522122 AK58-F 5'AAGAGAGGGGAGAGCTGGA  -3' AK58-R 5' CAGCTCTGAAAACATGAAT-3' 425 55 BstNI NEB2  BSA   60C  2% gel 1            2            1      
KT-L376 L376 IX 11 TGG_994720 682530-683405 L376 994720F 5'-AAGCTTCTCAGTCCTTCTGCAGCTG-3' L376 994720R 5'-GGATCTTCTACGACACCATCTGCAAA-3' 875 62 EcoR V NEB3  BSA   37C  1% gel 1            1            2      
AK59 RC17 IX 12 TGG_994720 1031692-1032094 AK59-F 5'TGAAGGAGTTGAAGAAGGAA -3' AK59-R 5'TGTATCTGTTCACACAGACCTC-3' 402 55 BsaI NEB4            50C  2% gel 1            2            1      
KT-L62A 62B, L62A IX 13 TGG_994720 1215768-1216254 KT-62B F 5’-TTAGACGCGAGAGAGCGGAAGTCTG –3’ KT-62B R 5’-GGTAGAACCCTCACTGAATGATGCCA-3’ 485 62 BsaJ I NEB2  BSA   60C  2% gel 1            2            1      
AK123   IX 14 TGG_994720 1600686-1601027 AK123F   5'-GCGAAGACGTCCTCTGTAT-3' AK123R   5'-CGACGAGAAGAGGAAGAG-3' 342 55 SfANI NEB3            37C  3% gel 1            1            2      
AK60 RC18 IX 15 TGG_994720 1638973-1639169 AK60-F 5' AATGCGTCAACGGGGAAA -3' AK60-R 5'GCACTTCATACAGTGGGATTTCT-3' 200 55 MnII NEB2  BSA   37C  2% gel 1            2            1      
AK124   IX 16 TGG_994720 1906146-1906530 AK124F   5'-GAAGATGTGATTCCAGAACG-3' AK124R   5'-AACTCCGGATACACAGCTC-3' 385 55 HpyCH4 III NEB4            37C  3% gel 1            2            2      
MIC1   IX 17 TGG_994720 2139758-2140314 MIC1-F  5'- AGCGTTGGTCAAGCATCTGT-3' MIC1-R  5'- TGCGTTTTTGTGTGTTGGTT -3' 557 55 Hha I NEB4  BSA   37C  2% gel 1            2            2      
SAG5C   IX 18 TGG_994720 2398736-2399264 NSAG5S     5’-TGCGGCAGCAGGAAATA-3’ NSAG5AS  5’-TCCGTACGCGCAAATGA-3’ 529 55 Hind III NEB2            37C  2% gel 1            1            2 Nci I NEB4            37C  2% gel 1            1            2
AK93 TGG_995354-D IX 19 TGG_995354 156485-157024 TGG_995354-DF  5'-AACGTGTGTGCAGTCTGG-3' TGG_995354-DR  5'-CCACCCGAAATCCAAGAG-3' 540 55 Cae8I NEB3            37C  3% gel 1            2            1      
AK92 TGG_995354-C IX 20 TGG_995354 128700-129372 TGG_995354-CF  5'-GTTTTTGCGTTTGCAGAG-3' TGG_995354-CR  5'-GTCTACCACAACACCGACTT-3' 672 55 SfcI NEB4  BSA   37C  3% gel 1            2            2      
B1.c   IX 21 TGG_995305 221-751, TGG_995301 1125-1655 multiple B1-S2  5’-TCTTCCCAGACGTGGATTTC-3’ B1-AS2  5’-CTCGACAATACGCTGCTTGA-3’ 531 55 Pml I NEB1  BSA   37C  2% gel 1            2            2      
AK125   IX 22 TGG_995301 290323-290755  AK125F  5'-GGCTATGTGCAAAAAGAGTT-3' AK125R  5'-TTGCACACAAAAGGTGGT-3' 433 55 Aci I NEB3            37C  3% gel 1            2            2      
AK126   IX 23 TGG_995301 732081-732525 AK126F  5'-CAGAATCCGAAACTGGTTAT-3' AK126R  5'-AGCACAAGTCAGACACACCT-3' 445 55 AlwNI NEB4            37C  3% gel 1            2            2      
AK127 AK127B IX 24 TGG_995301 1001173-1001758 AK127BF  5'-TCGTTTTGAGTTGCGTTATT-3' AK127BR  5'-CGAGAAATGGTGGTGAGA-3' 586 55 RsaI NEB1            37C  3% gel 1            2            2      
ST2A   V 1 TGG_994712 852-1586 ST2A-F 5'-GCTGGTGAAGAATAGGACAG-3' ST2A-R 5'- GTTTTGTCGCCTGAGCTT -3' 734 55 AciI NEB3            37C  2% gel 1            2            1      
ST2B   V 2 TGG_994712 46040-46758 ST2B-F 5'-ACCATTTCGGTGGATAAAC -3' ST2B R 5'-ACAGAGGACGAGGCAGAG -3' 718 55 MlnI NEB2  BSA   37C  2% gel 1            2            1      
KT-850 850 V 3 TGG_995288 153714 - 154480 850F   5'-GAGGACCTGGTAACGGCCCTCG-3' 850R   5'-CCGTCAAGGCTTGGATGTTTCGG-3' 767 62 SfaNI NEB3            37C  3% gel 1            2            1 Sau 96I NEB4            37C  3% gel 1            2            3
AK22 6xTGG9917_499-684 V 4 TGG_995288 267583-268182 TGG9917_449-684F 5’-GGCAACAAGATCCAGTTCGT  -3’ TGG9917_449-684R 5’- CAGTTTTCTGCATCCACGAG -3’ 600 55  HaeIII NEB2            37C  2% gel 1            2            1      
ST4B   V 5 TGG_994789 942-1515 ST4B-F 5'-CCTTGCAGGTTCAGATTTAG -3' ST4B-R  5'-ACTCTGCGTGGTATGTACG -3' 573 55 MboI NEB3            37C  2% gel 1            2            2      
ST3B   V 6 TGG_994803 1050-1721 ST3B-F 5'-CCCTTGACTCTGGTATCTCA -3' ST3B-R 5'-GAGAGTCAGCTGCCTTGG -3' 671 55 FokI NEB4            37C  2% gel 1            2            2      
AK121 AK121C V 7 TGG_995283 27633-28192 AK121CF 5'-CTGGCAACCATGTTCTTTAG-3' AK121CR 5'-CATCAGCCGTTCAGAGAC-3' 560 55 NlaIV NEB4  BSA   37C  3% gel 1            2            1      
AK100   V 8 TGG_995283 391757- 392103 AK100AF-5’TCTCTTGACTCCTGATCCAC-3’ AK100AR-5’CATGCGTTTTCTCCTCAG-3’ 347 55 PfIFI NEB4  BSA   37C  3% gel 1            2            1      
AK24 6xTGG9799-5 V 9 TGG_995283 798492-797772 TGG9799-5F 5’-CTCGCTTTGGGAGTGAGTTC-3' TGG9799-5R 5’-TTTTTGCAGTGTGCTTCCAG-3' 728 55 PCR                             3% gel 1            2            2      
AK101 AK101A V 10 TGG_995373 1719693-1719994 AK101AF 5'-CGTCTCTGATCCACATTAGG-3' AK101AR 5'-CCGCAGGAAACTGAAGTG-3' 302 55 HpaII NEB1            37C  3% gel 1            2            1      
KT-L354 L354 V 11 TGG_995373 1467130-1467729 KT-L354 F  5’-GGAGCGGTAGTGCTGTCAGCTGTAC-3’ KT-L354 R  5’-CTTTGTCTTGGATGCCTCCCTGTAAA –3’ 600 62 BsaJ I NEB2  BSA   60C  2% gel 1            2            1      
AK14   V 12 TGG_995373 1455698-1456345 AK14F  5'-TGTATTTCCAACACAAACGA-3' AK14R  5'-CGCTCGAAGAGAAAACACTA-3' 648 55 HpaII NEB1            37C  3% gel 1            2            1      
L358   V 13 TGG_995373  966472-966888 L358-F2  5’-AGGAGGCGTAGCGCAAGT-3’ L358-R2  5’-CCCTCTGGCTGCAGTGCT-3’ 418 55 Hae III NEB2            37C  2% gel 1            2            2 Nla III NEB4  BSA   37C  3% gel 1             2            1
KT-cA5 cA5 V 14 TGG_995373 775053 - 775902 CA5 995373F 5'-TGCACACGCGTCTTCACATACAGG-3' CA5 995373R 5'-GTCAACAATCGCGTTGGGATTTTC-3' 849 62 BsiE1 NEB4            60C  3% gel 1            2            1      
cS1-13 cS1 V 15 TGG_995373  601741-602159 cS1-13F   5’-CCCTGAATGTTCTTGTTTT-3’ cS1-13R   5’-GCCATATTGAGGCGTCAGGT-3’ 419 55 AlwN I NEB4            37C  3% gel 1            2            1    
1            2         1		 Bsm I NEB2            65C  3% gel  
AK25  6xTGG9861-5 V 16 TGG_995373 405880-406517 TGG9861-5F 5’-GTTCTCGTCGTTGTCTCTTG -3’ TGG9861-5R 5’-CGTTTTCAGTGTTTTCTTGG -3’ 638 55 FokI NEB4            37C  3% gel 1            2            1      
AK102 AK102D V 17 TGG_995373 117482-117871 AK102DF 5'-GCGACATACGCCAGAGTC-3'  AK102DR 5'-GTCCGTACCACACACACAT-3' 390 55 BccII NEB1  BSA   37C  3% gel 1            2            2      
AK70 RC24 VI 1 TGG_995364 88198-88530 AK70F-5'TTTTCTCTTGGCAGGAAATA-3' AK70R-5' CGAACATCTGAGAAAAGTCC-3' 332 55 NlaIII NEB2            37C  2% gel 1            2            2      
XM95   VI 2 TGG_995364 719197-719520 XM95-F  5'- CGCCAAGCTAGGACAGAGAC-3' XM95-R 5'- AGTCCTACATTGCCGTCACC -3' 324 60 Nla III NEB4  BSA   37C  2% gel 1            2            2      
AK72 RC25 VI 3 TGG_995364 1037548-1038005 AK72F-5'AGTCCCAGTGTAGGGTCAG  -3' AK72R-5' TGCAACGTCTGTCTTCTTC-3' 458 55 TaqI NEBU            65C  2% gel 1            2            2      
KT-950 950 VI 4 TGG_995364 1059246-1060081 KT-950 F  5'-CTGATTGGAACGTCCGGCTACATG-3' KT-950 R  5’-CCTGGGTTGTCTTCCCGGACAAT-3’ 836 62 Bsr I NEB3  BSA   37C  2% gel 1            2            2      
AK73 RC26 VI 5 TGG_995364 1458924-1459172 AK73F-5'CGTAGAGAACGACATGTTCA -3' AK73R-5'  ATCTATGTACAGGCGCAGTT-3' 253 55 PstI NEB3            37C  2% gel 1            2            2      
AK36 6xTGG9798-3 VI 6 TGG_995364 2018413-2019095 TGG9798-3F 5’-TCTTCCCAGTTGTGATGTTC -3’ TGG9798-3R 5’- ATCCCGATAAAACTCTGCTC -3’ 683 55  FokI NEB4            37C  3% gel 1            2            2      
PK1   VI 7 TGG_995364 2652219-2653121 PK1-F  5'- CGCAAAGGGAGACAATCAGT -3' PK1-R  5'- TCATCGCTGAATCTCATTGC -3' 903 55 Ava I NEB4            37C  2% gel 1            2            1 Rsa I NEB            37C  2% gel 1           2            2
L53   VI 8 TGG_995364  2962791-2963216 L53-F3  5’-CTCGTTGCGTTCCTTTGACT-3’ L53-R3  5’-GGAGAGAAGCTCGCAGTGTC-3’ 426 55 Hae III NEB2            37C  2% gel 1            2            1 HpyCH4 V NEB4            37C  2% gel 1            2            2
AK117   VI 9 TGG_995364 3548099-3548757 AK117B-F 5'-CTGCTTGAAATGCTTTCTCT-3 AK117B-R 5'-GATGCAGGCTCAACAGAC-3' 657 50 BsaJI NEB2            60C  2% gel 1            2            2      
SAG4   VIIa 1 TGG_995371 203841-204158 SAG4S1  5’-TACGATTTCAAGAAGGCGCT-3’ SAG4AS1  5’-GTTCTTTCCCGTGTCCTTGA-3’ 318 55 Taq I NEBU  BSA   65C  2% gel 1            1            2      
Bs328 L328 VIIa 2 TGG_995371 94791-95224 BS328-F2  5’-TCACTTTTTGAAGCGCTTGA-3’ BS328-R2  5’-CGTTCTTGTGCATGTTCTCGA-3’ 435 55 Dde I NEB3            37C  2% gel 1            1            2      
M95   VIIa 3 TGG_995300  129424-129640 MS95-1F  5’-CTGCAACAAACCATGACAGC–3’ MS95-4R  5’-TGGACTTCTTCGTCTCTCACC-3’ ~217-450 55 MicroS                             2% gel 1            1            2      
CS1  3xTGG2636 VIIa 4 TGG_995300 133031-133428 TGG2636-F2  5'- GTGTTTGGAGGACCAGCACT -3' TGG2636-R2  5'- GGCACTCCTCTCTGTTCGTC -3' 398 60 Alu I NEB2            37C  2% gel 1            1            2      
CS4 6xTGG9834-603 VIIa 5 TGG_995300 207764-208193 95051798-603F: 5'- CTCTTCTCTCCTCCGGACCT -3' 95051798-603R:  5'- TGGAAAGGCTAATGGAGTGG-3' 430 59 Nci I NEB4            37C  3% gel 1            2            1 Hha I NEB4  BSA   37C  2% gel 1            2            1
AK28 6xTGG9453-5 VIIa 6 TGG_995279 3327409-3328081 TGG9453-5F 5’-CGAGTACAAACTCGTCAAGG-3’ TGG9453-5R 5’-GAAAAGAGAGAAGGGTGGAA-3’ 673 55  BstUI NEB2            60C  3% gel 1            2            1      
CS12   VIIa 7 TGG_995279 3325398-3325874 100108051F 5’- CCGCGTTACTCAAGTTTCGT  -3’ 100108051R 5’- CATGCTCTGCCTTTTTCCTC –3’ 477 55 Hae III NEB2  BSA   37C  2% gel 1            2            2      
CS2 6xTGG9453-309 VIIa 8 TGG_995279 3018931-3019342 95059398-309F:  5'-TCGGATTAGTGTTGCAGGTG-3' 95059398-309R:  5'-GAAAAGCGTGAAACGGACTC-3' 412 59 Cvi JI 10x Cvi JI buffer + 10% DMSO  37C  3% gel 1            1            2 ApaLI  NEBU  BSA   37C  2% ge 1            2            1
CS11   VIIa 9 TGG_995279 2863606-2864100 95053274F 5'- ATGCCGTCAGGGTCTTTACA  -3' 95053274R 5'- CCCTCTGGATCACACGAGTT -3' 495 55 Rsa I NEB1            37C  2% gel 1            2            2      
CS5 6xTGG9803-628 VIIa 10 TGG_995279 2831433-2832061 95060335-628F:  5'-ACCGTAGTTCACCCGATCAA-3' 95060335-628R:  5'-CGCCATAGACAAGAGGAAGG-3' 629 55 Hae III NEB2            37C  2% gel 1            2            1      
CS10   VIIa 11 TGG_995279 2823064-2823553 95052105F 5'- GGGAGAGGGACACAGATTCA -3' 95052105R 5'- CTGCGCAAACACTGTAGGAA -3' 490 55 SacII NEB4  BSA   37C  2% gel 1            1            2      
CS3 6xTGG9803-695 VIIa 12 TGG_995279 2666751-2667307 95056843-695F:  5'-AGCGGATTTCCAACACTGTC -3' 95056843-695R:  5'-CTGCTGCATTCACAAACTCC-3' 557 55 Nla III NEB4  BSA   37C  2% gel 1            2            1 Mbo I NEB3            37C  2% gel 1            1            2
CS6   VIIa 13 TGG_995279 2527035-2527588 VIIROP-F 5'- TGAATTAGGCCTGGTCGTTC -3' VIIROP-R 5'- CGTTCCCCTCTTCATTTTGA -3' 554 55 Hha I NEB4  BSA   37C  2% gel 1            2            2      
MIC11   VIIa 14 TGG_995279 2310767-2312021 MIC11-F 5'-TGCAGCTCAAGAAGCTTTCA-3' MIC11-R 5'-ATTGTTTCCGCTTCAACCAG-3' 1255 63 TaqI NEBU  BSA   65C  2% gel 1            1            2      
CS7   VIIa 15 TGG_995279 2287108-2287552 VIIHSP-F 5'- CGTCTCTGGAGTCCTTCGTC -3' VIIHSP-R 5'- CCGAGGCTCATTTCTCTTTG -3' 445 55 Hae II NEB4  BSA   37C  2% gel 1            2            2      
AK43 RC5 VIIa 16 TGG_995279 2243569-2244368 AK43F 5' CATGCCCTTTTAAACGTAAG  -3' AK43R-5'CTGCACATCTGTATCCATTG-3' 786 53 EcoR1 NEB1            37C  2% gel 1            2            1      
CS8   VIIa 17 TGG_995279 2241513-2241918 VIIOWP-F  5'- GTTCCCCAACGCTAGTTTGA -3' VIIOWP-R  5'- GCGGAAACAAAATGAGCAAT -3' 406 55 Hpa I NEB4  BSA   37C  2% gel 1            1            2      
CS9   VIIa 18 TGG_995279 2197552-2197964 VIISUL-F   5'- GCCTTGTACACCGACACAAA  -3' VIISUL-R 5'- GTGAATGAGAAGGCGAGGTC -3' 413 55 BssSI NEB3  BSA   37C  2% gel 1            2            1      
TgSUB1   VIIa 19 TGG_995279   2006447-2006864 Sub3-F 5'-GATGCCCTCACTCTCCTCTC-3' Sub3-R 5'-CGTTCGTGGAGACGATAAGC-3' 418 55 HpyCH4 IV NEB1            37C  2% gel 1            2            1      
cS10-A6 cS10A VIIa 20 TGG_995279 2002567-2002907 CS10-A6F  5'-CTGGTTACATTTTCGCCTATCA-3' CS10-A6R  5'-CCTAGTCCAAACTAGGGCTTGA-3' 341 55 Rsa I NEB1            37C  2% gel 1            1            2 HpyCH4 IV NEB1            37C  3% gel 1            2            1
AK109 AK109A VIIa 21 TGG_995279  1488623-1489479 AK109AF  5'-CGTTTCGATTTTTCAAGAGA-3' AK109AR  5'- GTTTGTATGTATGCATGGATGT-3' 857 55 BfrBI NEB4  BSA   37C  3% gel 1            2            1      
M102   VIIa 22 TGG_995279  914651-914815 102-1F 5’-CAGTCCAGGCATACCTCACC-3’ 102-2R 5’-CAATCCCAAAATCCCAAACC-3’ ~166 55 MicroS                             3% gel 1            2            3      
AK44 RC6 VIIa 23 TGG_995279 768274-768932 AK44-F 5'- GTTTGGTTTCGATTCACTGT-3' AK44-R 5'- CGATTCGAAGACAAGACATT-3' 659 53 Nae I NRB1            37C  2% gel 1            2            1      
MIC2   VIIa 24 TGG_995279 248888-249605  MIC2-F1 5’- TGTGCCGAAGCTAGTGATTG –3’ MIC2-R1 5’- GACAGTGCAGTCGCTCCAT –3’ 718 55 Xmn I NEB2  BSA   37C  2% gel 1            2            1      
AK110   VIIa 25 TGG_995372 86114-86396 AK110BF 5’- TAGACATTTCTGCCGGTCT-3’ AK110B R 5’- AGAAGAAGAGGACGAAGAGG-3’ 283 55 BccI NEB3  BSA   37C  2% gel 1            2            2      
AK82 TGG_995372-A VIIa 26 TGG_995372 375219-375622 TGG_995372-AF  5'-AAAAAGATGTGCAACAGAGC-3' TGG_995372-AR  5'-CACTTTGGGATATGTTCGTT-3' 404 50 NlaIII NEB4  BSA   37C  3% gel 1            2            1      
AK29 6xTGG9870-5(2) VIIa 27 TGG_995372 469648-470408 TGG9870-5(2)F  5'-GCGATCAGACATTTAACGAG-3' TGG9870-5(2)R  5'-GGTCTAATGTGTACGCAAGC-3' 761 55 SpeI NEB2  BSA   37C  3% gel 1            2            1      
KT-c16AR c16A VIIa 28 TGG_995372 476283-476967 KT-C16R F2  5’-GGCTGCTCGAATTGGATTATCTGTGC –3’ KT-C16R R2 5’-GCCACATGGCCACGCCTCT –3’ 685 62 Sac II NEB4  BSA   37C  2% gel 1            2            1      
KT-c20B c20B VIIa 29 TGG_995372 481136-481800 KT-C20B F  5’-TGTGGTATTTGTTGCTCCGGACAA-3’ KT-C20B R  5’-AGCGGCGTCTGATCCTGCAG-3’ 665 62 BseR I NEB2  BSA   37C  2% gel 1            2            2      
AK103 AK103A VIIb 1 TGG_995366  4996718-4997122 AK103AF 5'-CATTAAACGTGTCTTTCACTG-3' AK103AR 5'-CCTGTGGTATCATCTGTCC-3' 405 55 ApaLI NEB4  BSA   37C  3% gel 1            2            1      
L339   VIIb 2 TGG_995366 4466260-4466902 L339-F  5’-AAGCTTTGCGAGCAAAACCA-3’ L339-R  5’-ATTCGCGTCCAAGTGAATTG-3’ 644 55 Hpa II NEB1            37C  2% gel 1            1            2      
L363   VIIb 3 TGG_995366 4158329-4158833 L363-F1  5’-GGCTATTCGGCAAACAACAC-3’ L363-R1 5'-GCAATCCAGTGAGTCACCAA-3' 504 55 Hpa II NEB1            37C  3% gel 1            2            2 HpyCH4 IV NEB1            37C  3% gel 1            1            2
AK104   VIIb 4 TGG_995366 3603126-3603586 AK104F   5'-TTTTTGTTTGATGTATTTGTAGA-3' AK104R   5'-GGTACGGCCACAACAGAC-3' 461 55 Tsp45I NEB1            65C  3% gel 1            2            2 BstXI NEB3            55C  3%gel 1            2            1
AK105   VIIb 5 TGG_995366 2892714-2893416 AK105F   5'-GTCGCTCTGTTGCGTGAA-3' AK105R   5'-CATCTATACCGTGCAGCTCT-3' 703 55 FokI NEB4            37C  3% gel 1            2            2 HhaI NEB4  BSA   37C  3%gel 1            2            1
cA5-2   VIIb 6 TGG_995366 2497338-2497768 cA5-2F   5’-CGAGTTGCAGCAAATCGATA-3’ cA5-2R   5’-TCGCTTTAAACTCGGGTGAG-3’ 431 55 Hga I NEB1            37C  2% gel 1            1            2      
AK106 AK106A VIIb 7 TGG_995366 2022315-2022654 AK106AF 5'-GCTCTCTTTGAGTGCGACAT-3' AK106AR 5'-AGAGGAAAAGATTTTGCTGAA-3'  340 55 AluI NEB2            37C  3% gel 1            2            2      
AK107 AK107B VIIb 8 TGG_995366 1613966-1614295 AK107BF 5'-GAAGATGAAGTGGCGTGT-3' AK107BR 5'-GGCAACACGGAAATAACATA-3' 330 55 HhaI NEB4  BSA   37C  3% gel 1            2            2      
AK108 AK108A VIIb 9 TGG_995366  973732-974085 108AF 5'-CTCTAGCGACGCAGAAGC-3' 108AR 5'-GCACAGGAAAGAAAAATGTC-3' 354 55 PshaI NEB4  BSA   37C  3% gel 1            2            2      
AK26 6xTGG9444-3 VIIb 10 TGG_995366 563365-564010 TGG9444-3F 5’-GGGAGCATGTTCTTCTCTCT-3' TGG9444-3R 5’-TGTCAGTCAATCAAACGTGA-3' 659 55 AfI III NEB3  BSA   37C  3% gel 1            2            2      
AK27 6xTGG9444-5 VIIb 11 TGG_995366 260328-260976 TGG9444-5F 5’-CTACGATGTGAAGAGGAACG -3’ TGG9444-5R 5’-CATGTATCCCGCAGTACAAT -3’ 649 55 ApaLI NEB4  BSA   37C  2% gel 1            2            2      
AK46 RC7 VIII 1 TGG_995362 110295-110678 AK46F- 5'CTGTCGAGACTGGATTTCTT -3' AK46R 5'  GGAGTACTTCAGCGTGTTCT-3' 384 53 PvuII NEB2            37C  2% gel 1            2            2      
AK47 RC8 VIII 2 TGG_995362 467431-467733 AK47F5'- GGACCAACACCGTCAATA -3' AK47F5'-  CACCCTCGAGTCTGTTCA-3' 300 53 BsaHI NEB2            60C  2% gel 1            2            2 MwoI NEBU        60C  3% gel  
AK111   VIII 3 TGG_995362 1004036-1004495 AK111BF-5’- CGGTAGCATTCGGTAGTAGT-3’ AK111BR-5’- TTGTATCAACGGTATTGCAC-3’ 460 55 PvuI NEB4  BSA   37C  2% gel 1            2            2      
AK112   VIII 4 TGG_995362 1513413-1513864 AK112F-5’-AAGAAATCTCACCTGGACAA-3' AK112R-5’-GTTTTATTGTGGATCCAAGG-3' 452 55 StyI NEB3  BSA   37C  2% gel 1            2            2      
AK162   VIII 5 TGG_995362 1658055 -1658403 AK162F    5'-CTAGACACCTTCTCCGTCTC-3' AK162R    5'-CTCATCTCGGGGATTTTC-3' 349 55 Mwo I NEB3            60C  3% gel 1            1            2      
AK152 AK152B VIII 6 TGG_995362 1773889-1774238 AK152BF 5'-CTGTCTGTCACTTGAAAACG-3' AK152BR 5'-GCTGTGAGCAGTAAGAGCTG-3' 350 55 HphI NEB4            37C  3% gel 1            2            2      
AK49 RC9 VIII 7 TGG_995362 2026084-2026428 AK49F-5' CGTCACGTCATCGTTATTC AK49R-5' ATCATGGCCACCTTCTTT-3' 350 55 HinfI NEB2            37C  2% gel 1            2            2      
SRS1   VIII 8 TGG_995362 2658988-2660373 SRS1-S1     5’- TTGTGCCTGAGTTCGTCTTG-3’ SRS1-AS1  5’- TGTCCACTCACGTACCGAAA-3’ 1386 55 ApaL I NEB4  BSA   37C  2% gel 1            2            2 Hae II NEB4  BSA   37C  2% gel 1            1            2
SAG1.c   VIII 9 TGG_995362   2662455-2662801 SAG1-S2  5’- CAATGTGCACCTGTAGGAAGC -3’ SAG1-AS2  5’- TTATCTGGGCAGGTGACAAC -3’ 347 55 Hae II NEB4  BSA   37C  2% gel 1            2            2 Sau96 I NEB4            37C  2% gel 1            2            2
SRS2   VIII 10 TGG_995362 2669450-2670581 SRS2S 5’-CGAGAATGGCGACGCGTGCGTCTT-3’ SRS2AS  5’-TTCCCCACTCAATAGGCAAGT-3’ 1132 55 Sfa NI NEB3            37C  2% gel 1            2            2      
AK113   VIII 11 TGG_995368 3811217- 3811796 AK113AF-5’TTCTTTCTGTTGCTCACCTC-3 AK113AR-5’GTTCGTTTTCTTCTCCACAC-3’ 580 55 NlaIII NEB4  BSA   37C  3% gel 1            2            2      
AK50 RC10 VIII 12 TGG_995368 3586009-3586358 AK50-F 5'-GCTGCACATCTTTTCTTTTT -3' AK50-F5'- GACACAAGGGAAGGGAAG-3' 350 55 BssHII NEB3            37C  2% gel 1            2            2      
AK51   VIII 13 TGG_995368 3050117-3050467 AK51F     5'-GGTGTGCCATATTTCTTTCT-3' AK51R     5'-CGGTCTCAGGAACTGTTTT-3' 351 55 DdeI NEB3            37C  3% gel 1            2            2      
AK52 RC11 VIII 14 TGG_995368 2494691-2495449 AK52-F 5'- TTCTGTGAGTGTGCTCTTTG-3' AK52-R5'- GGCACTTCGAGAAGAAAAG-3' 759 55 FokI NEB3            37C  2% gel 1            2            1      
AK30 6xTGG9416-5 VIII 15 TGG_995368 2298591-2299099 TGG9416-5F 5’-CGGGACCTTGTTTGTGACTT-3’ TGG9416-5R 5’- TACTAGCGGGCAGAGTCGAT -3’ 653 55 BsII NEB3            55C  3% gel 1            1            2      
SAG2   VIII 16 TGG_995368 1998357-1998902 SAG2-Fa  5’-ACCCATCTGCGAAGAAAACG-3’ SAG2-Ra 5’-ATTTCGACCAGCGGGAGCAC-3’ 546 55 Hinf I NEB2            37C  2% gel 1            1            2 Taq I NEBU  BSA   65C  2% gel 1            2            1
GRA1   VIII 17 TGG_995368 1445917-1446743 GRA1-5  5’-CGGTTTGCTTGTGTTGTTTG-3’ GRA1-3  5’- CATGGGGTACGATCACAACA-3’ 827 55 Hind III NEB2            37C  2% gel 1            2            2 Nla IV NEB4  BSA  37 C 2% gel 1            2            1
MAG1   VIII 18 TGG_995368 1444728-1445191 MAG1-F1   5’-TCTGTGTGCTAGACGTACTCTAACG-3’ MAG1-R1  5’-TTTCTGTTGCCTCCCCTGTT-3’ 463 55 BsmFI NEB4  BSA   65C  2% gel 1            2            1      
KT-c13R c13 VIII 19 TGG_995368 801141 - 801814 995368 C13F 5'-GATCACACGTCAACGACAAATTACGC-3' 995368 C13R 5'-CCCGAAGACACACTTCTCAGGTGAG-3' 673 62 Fnu4H1 NEB4            37C  1%gel 1            2            2      
KT-c24A c24A VIII 20 TGG_995368 586515-587174 KT-C24A F2  5’-ACCGTCGAAGCTGAAAGGTTGTGG-3’ KT-C24A R2  5’-TTAATCGAAGACGTGGCGAAAAGG-3’ 660 62 Mly I NEB4  BSA   37C  2% gel 1            2            1      
AK53 RC15 VIII 21 TGG_995368 465077-465800 AK53-F 5'TTCGTCGCATACCCACAGTA -3' AK53-R 5' TCATCCCTAGAGCCTCCTCA-3' 724 55 DdeI NEB3            37C  2% gel 1            2            1      
AK114   VIII 22 TGG_995345 11699-12148 AK114C-F 5'CTCTCTCCCGCTTTTTGA-3' AK114C-R-5'-ACTCTCAAGAGTCTACAGTTCCA-3'  450 50 BccI NEB4  BSA   37C  2% gel 1            2            1      
RC2 TGG9840-328 VIII 23 TGG_995345 70883-71483 TGG9840_140-328F-GCTGCATTCATGAAAACACAA-3' TGG9840_140-328R- AGAAACCTGAATGGATTCACAGA-3' 600 55 HpyCH4V NEB1            37C  2% gel 1            2            1      
ST10A   X 1 TGG_995346 27673-28381 ST10A- F 5'-TTTGTGCTTAGTTCTTGAGATG -3' ST10A- R 5'-GGAGCAGTTCATCTGGAAAT -3' 708 55 RsaI NEB1            37C  2% gel 1            2            1      
AK62 RC20 X 2 TGG_995361 3764642-3764919 AK62F 5'ACGCTCTTTGAGGGTCAT-3' AK62R 5'-ACTAAACGGACGCAGTTCT-3' 278 55 Mse I NEB2            37C  2% gel 1            2            1      
AK128   X 3 TGG_995361 3622746-3623105 AK128F  5'-AAAAGAGAGGAACGCGAGA-3' AK128R  5'-CCTCTTTGTGTGGCGTATT-3' 360 55 Hpy99I NEB4            37C  3% gel 1            2            1      
IMC2   X 4 TGG_995361 3400245-3401067 IMC2-F 5'- TGAGAACCTGGGAGGTAGGA -3' IMC2-R 5'- GCAGGAAGCAAACGTAGACC -3' 823 55 Sfa N I NEB3            37C  2% gel 1            2            2      
AK129   X 5 TGG_995361  3183002-3183355 AK129F  5'-GCGAGGGTCTGAGTAAGTC-3' AK129R  5'-GCTCTAATCCGTAGACGAAC-3' 354 55 DdeI NEB2            37C  3% gel 1            2            1      
GRA2   X 6 TGG_995361 3051578-3052277 GRA2-F  5'-GCAACAAGAGCCAGAAGAAC-3' GRA2-R  5'-ACCAAAAACTTGCATCCCAG-3' 700 55 Mnl I NEB2  BSA   37C  3% gel 1            2            1      
GRA3   X 7 TGG_995361   2875364-2876184 GRA3-S2     5’- TACGCGTCGAGTAACCAGTG –3’ GRA3-AS3  5’ – AGAGACTGGCACGATGCTTT – 3’ 821 55 Nci I NEB4            37C  2% gel 1            2            1      
AK63 RC21 X 8 TGG_995361 2267133-2267580 AK63F-5'CTGTATACCATCGAGCACTG -3' AK63R5'-CTCCTTCTTCGGCTTCTC-3' 448 55 BsmI NEB2            65C  2%gel 1            2            1      
AK64   X 9 TGG_995361 1817067-1817596 AK64F    5'-AGAAATCGGCGTATGCTC-3' AK64R    5'-ATGCGGAGAAGTCTCGTAG-3' 530 55 BsmAI NEB3            55C  3% gel 1            2            2      
AK65   X 10 TGG_995361 1438652-1439202 AK65-F 5'GCCTCCGGTAGTATTTCTTT-3' AK65-R 5'TCAACGTTCCAGTTATGTCA-3' 551 55 BfaI NEB4            37C  2% gel 1            2            1      
SRS4   X 11 TGG_995361 1087731-1088664 SRS4-S1  5’-GTTCCCGAAAGCATGAAGAA-3’ UK3-FUS3 5’- GCggatccCGTCTGGACAGCTGAAAATT-3’ 934 55 Nci I NEB4            37C  2% gel 1            1            2 Acc I NEB4            37C  2% gel 1            1             2
AK33 6xTGG9403-5 X 12 TGG_995361 931965-932588 TGG9403-5F 5’-CTCCTGAGGTGCTTCTCCAC -3’ TGG9403-5R 5’- TGGGGATTCTTCAGTTCACC-3’ 624 55 HpycH4IV NEB1            37C  3% gel 1            2            2      
AK66   X 13 TGG_995361 488396-488772 AK66F    5'-TTGAACTGAGTGCGTAACTG-3' AK66R    5'-GAGCCACTTCACCCCTAC-3' 377 55 BstUI NEB2            60C  3% gel 1            2            1      
AK130   X 14 TGG_995361 70518-70982 AK130F  5'-CTCGTTTATCGGGGGTAG-3' AK130R  5'-GACTCGAAGCAGGACGAC-3' 465 55 DdeI NEB2            37C  3% gel 1            2            2      
AK131 AK131A X 15 TGG_995355 240108-240467 AK131AF 5'-CATATACGGTGCTACCTGCT-3' AK131AR 5'-ATGCTGACAGAAGTGACTGA-3' 360 55 Bsl I NEB2            55C  3% gel 1            2            1      
c19-TA3 c19 X 16 TGG_995355  59567-60007 c19TA3-F  5’ – CGGTCTCGTTGAAGTGTTTG – 3’ c19TA3-R  5’ – GCCACAGTACAGCGAGTTGA – 3’ 441 55 Hinf I NEB2            37C  2% gel 1            2            2 Bfa I NEB4            37C  2% gel 1            2            1
AK34 6xTGG9919-3 X 17 TGG_995363 1749-2426 TGG9919-3F 5’-TCCTTGCCATTCAAGAATCC-3' TGG9919-3R 5’-GCAACTTGAGGAGAGGCAAC-3' 678 55 RsaI NEB1            37C  2% gel 1            2            1      
AK153 AK153C X 18 TGG_995363 280870-281169 AK153CF 5'-GCGTTCAACCAGGATCTC-3' AK153CR 5'-CACAAGACGCGAATCAAG-3' 300 55 Aci I NEB3            37C  3% gel 1            2            1      
AK32 6xTGG9919-5 X 19 TGG_995363 598940-599687 TGG9919-5F 5’-GAGTGCTCCTTCTGGACCTG -3’ TGG9919-5R 5’- TATGCACGGTCAATCGTTGT-3’ 748 55 XhoI NEB2  BSA   37C  3% gel 1            2            1      
AK132 AK132B X 20 TGG_995363 1812735-1813183 AK132BF 5'-GTGTCTGGATCTTCCACTATG-3' AK132BR 5'-TCTTCTCTCACGAGGAACC-3' 449 55 Mbo I NEB3            37C  3% gel 1            1            2      
AK67   X 21 TGG_995284 1704-2056 AK67-F' GTGGAGCATTACAATCAGGT-3' AK67-R'AGTAACTAGTTTGAGGGAATGC-3' 353 55 BsrI NEB3            65C  2% gel 1            2            2      
L366   X 22 TGG_995284  495515-496057 L366-F2  5’-CAATAAAAGTGCCAAGAGGGA-3’ L366-R2  5’-TTCAGCAGGCAGTGGAACAA-3’ 544 55 HpyCH4 IV NEB1            37C  2% gel 1            1            2 Aci I NEB3            37C  3% gel 1            2            2
M2AP   X 23 TGG_995284 591678-592186 M2AP-F  5'- CACGGTTGTCCAAGTGTGAG -3' M2AP-R  5'- ACACAACAAGCACGGAACAA -3' 510 55 Bfa I NEB4            37C  3% gel 1            2            1      
AK154   X 24 TGG_995284  814011-814381 AK154F   5'-GTTTGTGATGCGTATGTGTG-3' AK154R   5'-ATAACTCCCGAAGGCGTA-3' 371 55 MseI NEB2  BSA   37C  3% gel 1            2            1      
AK157   X 25 TGG_995284 977100-977649 AK157F  5'-AACCTCCACGTGTCTTTTC-3' AK157R  5'-GTAGCGCTGTTCAATCTCA-3' 550 55 HphI NEB4            37C  3% gel 1            2            2      
AK69 RC23 X 26 TGG_995284 1141166-1141655 AK69F-5'ACGAGCAACCATATCTTACC-3' AK69R-5'CGAACGGACAACAAGCTA 490 55 HinfI NEB2            37C  3% gel 1            2            3      
AK148 AK148B X 27 TGG_995297 625-1195 AK148BF 5'-GTGTTTTTGACCGGCTTT-3' AK148BR 5'-CAAGCTAATTACGTTTCTGTACC-3' 571 55 Hpy188III NEB4  BSA   37C  3% gel 1            2            1      
GRA6   X 28 TGG_995297  114937-115287 GRA6-F1x  5'- ATTTGTGTTTCCGAGCAGGT -3' GRA6-R1x  5’- TCGCCGAAGAGTTGACATAG -3' 351 55 Mse I NEB2  BSA   37C  3% gel 1            2            3      
RC4 6xTGG9393 257_262 X 29 TGG_995352 90448-91237 95058929_257_262F5'.-CGGACCTCAGAAAGCACATA -3' 95058929_257_262Rev.-GAGCAGTTGTCTTCGTCGTG -3' 601 55 Taq1 NEBU  BSA   65C  2% gel 1            2            1      
AK142 AK142A XI 1 TGG_995302 209266-209955 AK142AF 5'-AGGCGAAGTCACTCAGTTT-3' AK142AR 5'-GACGCTCGGATATCTGCT-3' 690 60 BstU I NEB2            60C  3% gel 1            2            2      
AK83 TGG_995302-B XI 2 TGG_995302 68301-68700 TGG_995302-BF  5'-CTCAGGTGTGCTCTGGAA-3' TGG_995302-BR  5'-ATAAATGGCTAACGCGATCT-3' 390 53 BsmI NEB2            65C  3% gel 1            2            2      
AK134   XI 3 TGG_995313 165435-165815 AK134F   5'-ATCCTGTTTACGGCGAAT-3' AK134R   5'-GATGCAATCAAGAGCACAAT-3' 381 55 Hpy188III NEB4            37C  3% gel 1            2            2      
AK143   XI 4 TGG_995313 269807-270263 AK143F   5'-AAATCCGACAGGCGTTCT-3' AK143R   5'-CAGTCGATTGATTGGGTTC-3' 457 60 AcuI NEB2  SAM   37C  3% gel 1            2            2      
AK133 AK133B XI 5 TGG_995313 315979-316348 AK133BF 5'-TAGACTGTGTTGAGCTGCTG-3' AK133BR 5'-TAGCGGGGTAGAGTATGAGA-3' 370 55 HaeIII NEB2            37C  3% gel 1            2            2      
ROP1   XI 6 TGG_995313  502540-502987 ROP1.F  5’-CGTGACATATACTGCACTGAC-3’ ROP1.R2  5’-CATCGTGAAACGTGTCATC-3’ 451 55 Hae III NEB2            37C  2% gel 1            2            2      
AK135   XI 7 TGG_995313 549309-549903 AK135F   5'-TCACAAGAATGGGACGTG-3' AK135R   5'-TGTTTATTTTATCGACAAGAGC-3' 595 55 BstF5I NEB4            65C  3% gel 1            2            2      
AK10   XI 8 TGG_995313 1086673-1087172 AK10F    5'-AAAACGCGTTAGGGTTTAG-3' AK10R     5'-ACGTGGCTGAGAGAAAAAG-3' 500 53 SfcI NEB4  BSA   37C  3% gel 1            2            2      
GRA4   XI 9 TGG_995313 1345008-1345627 GRA4-F  5'-CCATCCTCTGTTCAACCGGG-3' GRA4-R  5'-AGTGGCAAACGAGCACGACT-3' 620 55  Hga I NEB1            37C  2% gel 1            2            2      
AK141   XI 10 TGG_995313 1451742-1452112 AK141F   5'-GTATTTTTGATGCCGAGAAC-3' AK141R   5'-CCGGGTAAAGATAGATGTGA-3' 371 55 SpeI NEB2  BSA   37C  3% gel 1            2            2      
AK155B AK155B XI 11 TGG_995313 1609506-1610180 AK155BF 5'-CTGTTCAACAACGACCACT-3' AK155BR 5'-ACGTAACTCCCAGCTTCC-3' 675 55 HaeIII NEB2            37C  3% gel 1            2            1      
AK155A AK155A XI 12 TGG_995313  1609826 -1610400 AK155AF  5'-CAACGTTTCCCTGCAGTC-3' AK155AR  5'-CATCCGGTGAGCAGACAC-3' 575 55 HpyCH4 V NEB1            37C  3% gel 1            2            2      
PKG   XI 13 TGG_995313  1761933-1762760 cGMP-F 5'- CCTCTCTCGCACTCACTTCC -3' cGMP-R 5'- TCTATAGGCCGGAAAACACG -3' 828 60 Hinf I NEB2            37C  2% gel 1            2            2      
c1-24   XI 14 TGG_995313 1896275-1896678 C1-24F  5’ – ACAGCGACATCCAGATGTTC – 3’ C1-24R  5’ – GGAAAGCATGAGTGGTTACA – 3’ 405 55 Pci I NEB3            37C  2% gel 1            2            2      
UPRT   XI 15 TGG_995313 2474398-2474953 UPRT-F1  5’-CCCGATATTCGACAAACGAC-3’ UPRT-R1  5’-GAGCCGTCTGCTTCATGAGC-3’ 555 55 Bst UI NEB2            60C  2% gel 1            2            2      
AK137   XI 16 TGG_995313 2747444-2747815 AK137F    5'-AGAGGTGTACGTGATTCCTG-3' AK137R    5'-GTAGATGCCGACCAGAGC-3' 372 55 BseRI NEB2            37C  3% gel 1            2            2      
AK11   XI 17 TGG_995313 3084624-3085117 AK11F   5'-GTGTTTCTCTCGCAGTTTTT-3' AK11R  5'-GGCAAACTTAGTTCAACGAC-3' 494 53 HinFI NEB2            37C  3% gel 1            2            2      
AK138   XI 18 TGG_995313 3665884-3666333 AK138F    5'-CACCCGCAAGATACACCT-3' AK138R    5'-ATAGGCCGGGTAGAAAAAG-3' 450 55 Hinf I NEB2            37C  3% gel 1            2            2      
L344   XI 19 TGG_995313 3839949-3840436 L344-F   5’ – TCGCAACTGAAACCTGAAAA-3’ L344-Rx 5’ – CGTTGACTGAGTGGTGCAAA-3’ 491 55 Rsa I NEB1            37C  2% gel 1            2            2      
AK20 6xTGG9807-3 XI 20 TGG_995313 3958592-3959214 TGG9807-3’FTAGAGAGGCCATCCCATTTG-3' TGG9807-3’RCCTGCTACACGCCTCCTTAC-3' 623 50 ScrFI NEB4            37C  3% gel 1            2            2      
AK12   XI 21 TGG_995313 4420425-4421136 AK12F    5'-CCAACACAATTTTCCAGTTT-3' AK12R    5'-GCATAGCATGCTTGTTACAG-3' 712 53 BstNI NEB2  BSA   60C  3% gel 1            2            2      
AK139 AK139A XI 22 TGG_995313 4983182-4983551 AK139AF 5'-GAACTTACCTCACTTTCAGC-3' AK139AR 5'-GGAAAGAGACACGCGTTA-3' 370 55 HpyCH4 III NEB4            37C  3% gel 1            2            2      
AK140   XI 23 TGG_995313 5346644-5347016 AK140F   5'-TCTGTTTTCTGGGACGTG-3' AK140R   5'-GCCGCTCTTCTACTCAGC-3' 373 55 BstF5I NEB4            65C  3% gel 1            2            2      
AK149 AK149A XI 24 TGG_995285 915509-916197 AK149AF 5'-AGGACAAAGGGAATGACTG-3' AK149AR 5'-CAAACGCTTCAGTTGTGTAG-3' 689 55 BsmAI NEB3            55C  3% gel 1            2            2      
AK80 TGG995285-B XI 25 TGG_995285 711520-711769 TGG_995285-BF 5'-CTATGCCTACTTCCTTATTGCT-3' TGG_995285-BR 5'-CATTTTCCTCCAGACTGA-3' 250 53 Hae III NEB2            37C  3% gel 1            2            2      
AK81 TGG_995285-A XI 26 TGG_995285 674698-675059 TGG_995285-AF  5'-ATCAAATACGGCTGGTACAC-3' TGG_995285-AR 5'- ATTCCTCGAACAACACTGTC-3' 362 53 HphI NEB4            37C  3% gel 1            2            2      
AK37 6xTGG9388-5 XII 1 TGG_994756 327627-328374 TGG9388-5F 5’-CATCTCATGCTCGTTTCTTC -3’ TGG9388-5R 5’-TGGAACAGTATTTCGGTGTC-3’ 748 55 Sau3AI NEBU  BSA   37C  3% gel 1            1            2 AfI III NEB3  BSA   37C  3% gel 1            2            1
AK145   XII 2 TGG_994756 117582-117937 AK145F   5'-TAGTGCTGCATTCTTCTCTT-3' AK145R   5'-GTGCTTTGACGTTGACATT-3' 356 55 AfI III NEB3  BSA   37C  3% gel 1            1            2      
SAG3   XII 3 TGG_995306 140010-140320 P43S1  5’-CAACTCTCACCATTCCACCC-3’ P43AS1  5’-GCGCGTTGTTAGACAAGACA-3’ 311 55 Nci I NEB4            37C  3% gel 1            2            3      
AK78   XII 4 TGG_995287 1116198-1116787 AK78-F' CGTTTAGCGATATGAAGTGG-3' AK78-R 5'-CCAGTCTATACTTGTGACGGTA-3' 590 55 NlaIII NEB2  BSA   37C  2% gel 1            2            1      
AK156   XII 5 TGG_995287 731351-731778 AK156F   5'-ATCCAGGTTACCCGAACA-3' AK156R   5'-GTACGCGACTGCCTGCTAC-3' 428 55 Hpy188III NEB4  BSA   37C  3% gel 1            2            1      
M6   XII 6 TGG_995287 529076-529256 6-1F 5’-CAGAGATTCAACGAGCAACG-3’ 6-3R 5’-ACGCGATCAGTGGGTTTATG-3’ ~200 55 MicroS                             3% gel 1            2            1      
M144   XII 7     144-1F 5’-TTACCGTTCCAATCGTTTCC-3’ 144-7R 5’-ATGCGAATACATGTTGATGC-3’ ~180 55 MicroS                             4% gel 1            2            3      
MIC6   XII 8 TGG_995287  262905-263413 MIC6-F2  5'- TCCTGGGAATGGAGAGATTG -3' MIC6-R   5' TCCTATAATGGGGAGGCACA -3' 509 55 Pst I NEB3  BSA  37C  2% gel 1            2            1      
CRK2   XII 9 TGG_995287 71764-72349 Crk2-F2 5'- CAAAGTTCTCGGCACTCCTC -3' Crk2-R2 5'- TTTGGCACCTGACACCAATA -3' 586 55 Aci I NEB3            37C  2% gel 1            2            2      
AK91 TGG_995286-A XII 10 TGG_995286 167346-167688 TGG_995286-AF 5'-AGACTTCCTTCCCAGCAT-3' TGG_995286-AR 5'-GTTCCGTTAGCTGTCCTGT-3' 343 53 BfaI NEB4            37C  3% gel 1            2            2      
AK90 TGG_995286-B XII 11 TGG-995286 238414-238891 TGG_995286-BF  5'-GATAGCGACGAGGCATAAC-3' TGG_995286-BR  5'-GACACTTGGGAGGTAGCAT-3' 478 53 AccI NEB4            37C  3% gel 1            2            2      
AK115   XII 12 TGG_995286 464177-464876 AK115F   5'-CACACGCTTTTGACAAAGT-3' AK115R   5'-GGCAGACTTCTCCATACTGT-3' 700 55 BstU I NEB2            60C  3% gel 1            2            2      
AK74   XII 13 TGG_995291  5347-5813 AK74F    5'-ATAGAGCTCGAACACCGAGT-3' AK74R    5'-ATGCACAGTCACGACGTA-3' 467 55 BfaI NEB4            37C  3% gel 1            2            2      
L31-T7   XII 14 TGG_995291  677746-677973 L31-F2x   5'-CTCTCCCCGTTCCGAGAC-3' L31-R2x  5'-CGGGAAAAGCGAAGTGGT-3' 228 55 MicroS                             3% gel 1            2            2      
AK75   XII 15 TGG_995291 1155951-1156181 AK75-F'GACAGGAGCGCCTCAAAC-3' AK75-R'CGTTGACTTCCCATCTAAAG-3' 240 53 BsaHI NEB4  BSA   37C  3% gel 1            2            2      
AK116 AK116A XII 16 TGG_995291 1572029-1572398 AK116AF 5'-ACGCTGACCTGAAGTACTGA-3' AK116AR 5'-TCACGGATACTTTCTTCTCC-3' 370 50 HhaI NEB4  BSA   37C  3% gel 1            2            2      
KT-c18B c18B XII 17 TGG_995291 1920036-1920496 KT-C18 F2  5’-AGGATCTCGAGCCGCCG- 3’ C18 R  5’-AGTGTGTGCGACGTGGCTTCAGTCT-3’ 460 62 Bbs I NEB2  BSA   37C  2% gel 1            2            2      
L353.c   XII 18 TGG_995291 2095204-2095714 L353-F2 5’-TTTCCGACAAGGTGGAGAGC-3’ L353-R2 5’-AGAGCAGGAGGGATGCAGTG-3’ 511 55 Sau96I NEB4            37C  2% gel 1            2            2 Nla III NEB4  BSA   37C  2% gel 1            2            2
DHRTS   XII 19 TGG_995291 2107525-2107965 DHRTS-F 5’-CTGCCTCGACTCGGCCTATAGTCAG-3’ DHRTS-R 5’-ATAGAAACGCGGCACCATGC-3’ 435 55 BsmAI NEB3            55 C 2% gel 1            2            2      
AK165   XII 20 TGG_995291 2274142-2274496 AK165F  5'-AAATGGGAAATAAGGGAAAC-3' AK165R  5'-GCAGACTTTGCTACAGGACT-3' 355 55 Apo I NEB3  BSA   37C  2% gel 1            1            2      
TgCP1   XII 21 TGG_995291 2471168-2471569 TgCP1-F2 5'- GGTCGTTGAAGGCTTCTGTG -3' TgCP1-R2 5'- GAAAGAGACTGGCGAAGACG -3' 402 55 Rsa I NEB1            37C  2% gel 1            2            2      
AK   XII 22 TGG_995291  3036716-3037167 AK-F1  5’-TCTTGACCAGTTCAATCCGACTTCC-3’ AK-R1  5’-CAATGGCTCCCATGTACCCA-3’ 449 55 Nla III NEB4  BSA   37C  2% gel 1            2            2      
B10   XII 23 TGG_995291 3201947-3202397 B10-F 5’-TTGGAAGAAAGTCAGCGCAC-3’ DHRTS-R 5’-ATAGAAACGCGGCACCATGC-3’ 451 55 HpyCH4 IV NEB1            37C  2% gel 1            2            2      
KT-c20F c20 XII 24 TGG_995291 3333281-3334000 KT-C20F F  5’- CAGCTGACTACTAGAATCGCACGCG - 3’ KT-C20F R  5’-CCACTGTCCCGTCCGTGTCTTCTT-3’ 720 62 Bcc I NEB1  BSA   37C  2% gel 1            2            2      
AK77 RC27 XII 25 TGG_995299 1023070-1023322 AK77F-5'CTCTACGTCGCACAGAGC  -3' AK77R-5'   GCGAACAGTCACTAGGAAAC-3' 253 55 NlaIII NEB4  BSA   65C  2% gel 1            2            2      
M163   XII 26 TGG_995299 710726-710851 163-1F 5’-GCTTTTGACGTCCACTTCG-3’ 163-2R 5’CCTCCTTCTCAAAGGTCAAGC-3’ ~ 180 55 MicroS                             3% gel 1            2            3      
BM17053   XII 27 TGG_995299  419375-419631 BM17053-F 5'- CGACAAGTCCATGCGAACTA -3' BM17053-R 5'- GGCAACAGGAGGTAGAGGAG -3' 248-254       I/II,III 55 Hinf I NEB2            37C  2% gel 1            2            2      
MIC5   XII 28 TGG_995299 181308-182021 MIC5-F  5'- GCGGTGGTCAGATTCCTCTA -3' MIC5-R  5'- GCCCAGTGTCGATAGCAAAT -3' 714 55 Hha I NEB4  BSA   37C  2% gel 1            2            2      
ST5A   XII 29 TGG_994811 46445-47100 ST5A-F   5'-AACAGCGACGACCATAGAT -3' ST5A-R   5'-GTAGGGATGGTGCTGTCTC-3' 655 55 HaeIII NEB2            37C  2% gel 1            2            2      
ST5B   XII 30 TGG_994811 46050-46700 ST5B-F 5'-AACCGATCCATCCCTCTT -3' ST5B-R  5'-CCACCAATTCGATGTGCTT -3' 650 55 BsmAI NEB3            55C  2% gel 1            2            2      
ST6A   XII 31 TGG_995341 40903-41753 ST6A-F 5'-CCCTCTTCGACTTCTGTAAC -3' ST6A-R  5'-TTCTATGCGAACGTGTGAT -3' 850 55 HpaII NEB1            37C  2% gel 1            2            2      

Sibley Lab (sibley@borcim.wustl.edu)
Department of Molecular Microbiology
Washington University School of Medicine
St. Louis, MO USA

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