LOCUS       ToxoSuperC   7421 bp    DNA   circular             5-DEC-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|393096313|
COMMENT     VNTNAME|ToxoSuperCos|
COMMENT     VNTREPLTYPE|Cosmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTUDF|Cloned by:|1|Marc-Jan Gubbels|
COMMENT     VNTUDF|Form:|1|Cosmid Library|
COMMENT     VNTUDF|Freezer Box:|1|Cosmid RH|
COMMENT     VNTUDF|Freezer Position|1|Tower 3|
COMMENT     VNTUDF|Host strain:|1|XL1-Blue MRF'|
COMMENT     VNTUDF|Organism|4|E.coli|
COMMENT     VNTUDF|Selection:|5|Kamamycin|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #8=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 3779 0 4607 0 5343 0) 
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FEATURES             Location/Qualifiers
     misc_feature    1207..2308
                     /vntifkey="21"
                     /label=sCos\cloning\vector\cos-site\(1207-2308)
     misc_structure  complement(5417..7163)
                     /vntifkey="88"
                     /label=Expression\vector\pUK-S\609-2355,\KANr\(5417-7163)
     misc_difference 2319..5300
                     /vntifkey="85"
                     /label=DHFR-TS\(2319-5300)
     misc_feature    37..1189
                     /vntifkey="21"
                     /label=sCos\cloning\vector\cos-site\(37-1189)
     primer_bind     complement(72..91)
                     /vntifkey="28"
                     /label=T7\primer\(72-91)
     primer_bind     51..69
                     /vntifkey="28"
                     /label=T3\primer\(51-69)
BASE COUNT     1784 a      1898 c      1850 g      1889 t 
ORIGIN
        1 gttaacgcta gcatggatct cgggccccgt cttcaagaat tcgcggccgc aattaaccct 
       61 cactaaagga tccctatagt gagtcgtatt atgcggccgc gaattctcat gtttgaccgc 
      121 ttatcatcga taagctctgc tttttgttga cttccattgt tcattccacg gacaaaaaca 
      181 gagaaaggaa acgacagagg ccaaaaagct cgctttcagc acctgtcgtt tcctttcttt 
      241 tcagagggta ttttaaataa aaacattaag ttatgacgaa gaagaacgga aacgccttaa 
      301 accggaaaat tttcataaat agcgaaaacc cgcgaggtcg ccgccccgta acaaggcgga 
      361 tcgccggaaa ggacccgcaa atgataataa ttatcaattg catactatcg acggcactgc 
      421 tgccagataa caccaccggg gaaacattcc atcatgatgg ccgtgcggac ataggaagcc 
      481 agttcatcca tcgctttctt gtctgctgcc atttgctttg tgacatccag cgccgcacat 
      541 tcagcagcgt ttttcagcgc gttttcgatc aacgtttcaa tgttggtatc aacaccaggt 
      601 ttaactttga acttatcggc actgacggtt accttgttct gcgctggctc atcacgcagg 
      661 ataccaaggc tgatgttgta gatattggtc accggctgag ggttttcgat tgccgctgcg 
      721 tggatagcac catttgcgat caggcgtcct tgatgaatga cactccattg cgaataagtt 
      781 cgaaggagac ggtgtcacga atgcgctggt ccagctcggt cgattgcctt ttgtgcagca 
      841 gaggtatcaa tctcaacgcc aaggctcatc gaagcgcaat attgctgctc accaaaacgc 
      901 gtattgacca ggtgttcaac ggcaaatttc tgcccttctg atgtcagaaa ggcaaagtga 
      961 ttttctttct ggtattcagt tgctgtgtgt cggtttcagc aaaaccaagc tcgcgcaatt 
     1021 cggctgtgca gatttagaag gcagatcacc agacagcaac ggccaacgga aaacagcgca 
     1081 tacagaacat ccgtcgccgc gccgacaacg tgataatttt tatgacccat gatttatttc 
     1141 cttttagacg tgagcctgtc gcacagcaaa gccgccgaaa gttcctcgaa gctagcttca 
     1201 gacgtgtcta gatacgtctg ctttttgttg acttccattg ttcattccac ggacaaaaac 
     1261 agagaaagga aacgacagag gccaaaaagc tcgctttcag cacctgtcgt ttcctttctt 
     1321 ttcagagggt attttaaata aaaacattaa gttatgacga agaagaacgg aaacgcctta 
     1381 aaccggaaaa ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacaaggcgg 
     1441 atcgccggaa aggacccgca aatgataata attatcaatt gcatactatc gacggcactg 
     1501 ctgccagata acaccaccgg ggaaacattc catcatgatg gccgtgcgga cataggaagc 
     1561 cagttcatcc atcgctttct tgtctgctgc catttgcttt gtgacatcca gcgccgcaca 
     1621 ttcagcagcg tttttcagcg cgttttcgat caacgtttca atgttggtat caacaccagg 
     1681 tttaactttg aacttatcgg cactgacggt taccttgttc tgcgctggct catcacgcag 
     1741 gataccaagg ctgatgttgt agatattggt caccggctga gggttttcga ttgccgctgc 
     1801 gtggatagca ccatttgcga tcaggcgtcc ttgatgaatg acactccatt gcgaataagt 
     1861 tcgaaggaga cggtgtcacg aatgcgctgg tccagctcgg tcgattgcct tttgtgcagc 
     1921 agaggtatca atctcaacgc caaggctcat cgaagcgcaa tattgctgct caccaaaacg 
     1981 cgtattgacc aggtgttcaa cggcaaattt ctgcccttct gatgtcagaa aggcaaagtg 
     2041 attttctttc tggtattcag ttgctgtgtg tcggtttcag caaaaccaag ctcgcgcaat 
     2101 tcggctgtgc agatttagaa ggcagatcac cagacagcaa cggccaacgg aaaacagcgc 
     2161 atacagaaca tccgtcgccg cgccgacaac gtgataattt ttatgaccca tgatttattt 
     2221 ccttttagac gtgagcctgt cgcacagcaa agccgccgaa agttcctcga ccgatgccct 
     2281 tgagagcctt caacccagtc agctcctttt aattaagtct agcatgtcat tcgattttca 
     2341 ccccccgcgt agttcctgtg tgtcattcgt tgtcgagaca actctgtccc gccccggtgc 
     2401 tgttccatat gcgtgacttt cccgcaattt tttcagactt tcaggaaaga caggctccgg 
     2461 aacgatctcg tccatgactg gtaaatccac gacaccgcaa tggcccccag cacctctatc 
     2521 tctcgtgcca ggggactaac gttgtatgcg tctgcgtctt gtctttttgc attcgctttc 
     2581 caaaaaagag agccatccgt tcccccgcac attcaacgcc gcgagtgcgg tttttgtctt 
     2641 ttttgagtgg taggacgctt ttcatgcgcg aactacgtgg acattaagtt ccattctctt 
     2701 tttcgacagc acgaaacctt gcattcaaac ccgcccgcgg aagatccgat cttgctgctg 
     2761 ttcgcagtcc cagtagcgtc ctgtcggccg cgccgtctct gttggtgggc agccgctaca 
     2821 cctgttatct gactgccgtg cgcgaaaatg acgccatttt tgggaaaatc ggggaacttc 
     2881 attctttaaa agtatgcgga ggtttccttt ttcttctgtt cgtttctttt tctcgggttt 
     2941 gataaccgtg ttcgatgtaa gcactttccg tctctcctcc gtgctttgtt cgacatcgag 
     3001 accaggtgtg cagatccttc gcttgtcgat ccggagacgc gtgtctcgta gaaccttttc 
     3061 attttaccac acggcagtgc ggagcactgc tctgagtgca gcagggacgg gtgaagtttc 
     3121 gctttagtag tgcgtttctg ctctacgggg cgttgtcgtg tctgggaaga tgcagaaacc 
     3181 ggtgtgtctg gtcgtcgcga tgacccccaa gaggggcatc ggcatcaaca acggcctccc 
     3241 gtggccccac ttgaccacag atttcaaaca ctttcgtcgt gtgacaaaaa cgacgcccga 
     3301 agaagccagt cgcctgaacg ggtggcttcc caggaaattt gcaaagacgg gcgactctgg 
     3361 acttccctct ccatcagtcg gcaagagatt caacgccgtt gtcatgggac ggaaaaactg 
     3421 ggaaagcatg cctcgaaagt ttagacccct cgtggacaga ttgaacatcg tcgtttcctc 
     3481 ttccctcaaa gaagaagaca ttgcggcgga gaagcctcaa gctgaaggcc agcagcgcgt 
     3541 ccgagtctgt gcttcactcc cagcagctct cagccttctg gaggaagagt acaaggattc 
     3601 tgtcgaccag atttttgtcg tgggaggagc gggactgtac gaggcagcgc tgtctctggg 
     3661 cgttgcctct cacctgtaca tcacgcgtgt agcccgcgag tttccgtgcg acgttttctt 
     3721 ccctgcgttc cccggagatg acattctttc aaacaaatca actgctgcgc aggctgcagc 
     3781 tcctgccgag tctgtgttcg ttcccttttg tccggagctc ggaagagaga aggacaatga 
     3841 agcgacgtat cgacccatct tcatttccaa gaccttctca gacaacgggg taccctacga 
     3901 ctccgtggtt ctcgagaaga gaaggaagac tgacgacgca gccactgcgg aaccgagcaa 
     3961 cgcaatgagc tccttgacgt ccacgaggga gacaactccc gtgcacgggt tgcaggctcc 
     4021 ttcttcggcc gcagccattg ccccggtgtt ggcgtggatg gacgaagaag accggaaaaa 
     4081 acgcgagcaa aaggaactga ttcgggccgt tccgcatgtt cactttagag gccatgaaga 
     4141 attccagtac cttgatctca ttgccgacat tattaacaat ggaaggacaa tggatgaccg 
     4201 aacgggcgtt ggtgtcatct ccaaattcgg ctgcactatg cgctactcgc tggatcaggc 
     4261 ctttccactt ctcaccacaa agcgtgtgtt ctggaaaggg gtcctcgaag agttgctgtg 
     4321 gttcattcgc ggcgacacga acgcaaacca tctttctgag aagggcgtga agatctggga 
     4381 caagaatgtg acacgcgagt tcctcgattc gcgcaatctc ccccaccgag aggtcggaga 
     4441 catcggcccg ggctacggct tccagtggag acacttcggc gcggcataca aagacatgca 
     4501 cacagactac acagggcagg gcgtcgacca gctgaagaat gtgatccaga tgctgagaac 
     4561 gaatccaaca gatcgtcgca tgctcatgac tgcctggaat cctgcagcgc tggacgaaat 
     4621 ggcgctgccg ccttgtcact tgttgtgcca gttctacgtg aacgaccaga aggagctgtc 
     4681 gtgcatcatg tatcagcggt cgtgcgatgt cggcctcggc gtccccttca acatcgcttc 
     4741 ctattcgctt ttgacgctca tggttgcaca cgtctgcaac ctaaaaccta aggagttcat 
     4801 tcacttcatg gggaacacgc atgtctacac gaaccatgtc gaggctttaa aagagcagct 
     4861 gcggagagaa ccgagaccgt tccccattgt gaacatcctc aacaaggaac gcatcaagga 
     4921 aatcgacgat ttcaccgccg aggattttga ggtcgtgggc tacgtcccgc acggacgaat 
     4981 ccagatggag atggctgtct agcggaaata cagaagctgc ccgtctctcg ttttcctctc 
     5041 ttttcggagg gatcagggag agtgcctcgg gtcggagaga gctgacgagg gggtgccaga 
     5101 gacccctgtg tcctttatcg aagaaaaggg atgactcttc atgtggcatt tcacacagtc 
     5161 tcacctcgcc ttgttttctt tttgtcaatc agaacgaaag cgagttgcgg gtgacgcaga 
     5221 tgtgcgtgta tccactcgtg aatgcgttat cgttctgtat gccgctagag tgctggactg 
     5281 ttgctgtctg cccacgacag cagacaactt tccttctatg cacttgcagg atgaattctg 
     5341 cagcccgggg gatcgatcca ctagttctag tggtaccaag cttagatctc tcgagactag 
     5401 tagcggcctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat 
     5461 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag 
     5521 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata 
     5581 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta 
     5641 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga 
     5701 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat 
     5761 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa 
     5821 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct 
     5881 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga 
     5941 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag 
     6001 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa 
     6061 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct 
     6121 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga 
     6181 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca 
     6241 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca 
     6301 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag 
     6361 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagttt 
     6421 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 
     6481 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 
     6541 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 
     6601 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 
     6661 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 
     6721 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 
     6781 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 
     6841 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 
     6901 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 
     6961 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 
     7021 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 
     7081 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 
     7141 tctgtggata accgtattac cgctagccag gaagagtttg tagaaacgca aaaaggccat 
     7201 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc 
     7261 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac 
     7321 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag 
     7381 cctttcgttt tatttgatgc ctggcagttc cctactctcg c 
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